Electron microscopy of newly-discovered H-SC phages

As part of the culmination of the semester-long study by H-SC Molecular Biology students into the diversity and genetics of bacteriophages, the class recently took a field trip to the University of Mary Washington to utilize their electron microscope facility.  Mary Washington, like H-SC, is a member of the Howard Hughes Medical Institute’s Science Education Alliance, and UMW’s Dr. Kathy Loesser and Dr. Lynn Lewis generously shared their resources with the H-SC Molecular Biology class.

UMW's Dr. Loesser gives the class a tour of the electron microscope facility

UMW's Dr. Loesser gives the class a tour of the electron microscope facility

Electron microscopy can provide over 100,000-fold magnification, allowing for visualization of the viral participles that students isolated and purified over the course of the semester.

Carter Mavromatis '12 and Dr. Loesser observing "Fhageblaster", a phage isolated by Carter near his hometown of Virginia Beach

Carter Mavromatis '12 and Dr. Loesser observe "Fhageblaster", a phage isolated by Carter from Virginia Beach, VA

The electron microscope pictures provide valuable clues about the evolution of each individual phage and how they relate to phages isolated around the world by scientists at dozens of other colleges and universities.

"Arturo", isolated by Duncan Oliphant '12 near the H-SC campus

"Arturo", isolated by Duncan Oliphant '12 near the Sagebrook apartments just off the H-SC campus

VenaBlue, isolated by Sam Smith '13 near Venable Hall on the H-SC campus

"VenaBlue", isolated by Sam Smith '13 near Venable Hall on the H-SC campus

More Service from BIOL 108…

Several students from Dr. Goodman's Environmental Biology class cleaned up 
cigarette butts around fraternity circle on Thurs, Dec 1. Patrick Adams, 
George Parrish, John Parrish, Matthew Gates, Matt Vail, Ibn Salaam, and 
Jack Gibson (pictures below) each filled a sandwich bag full of butts- 
yummy! For more about the problem that cigarette butts pose as pollution, 
On Saturday, Dec 3, Colin Nickerson, Derrick Maxwell, and Patrick Lynch
(pictured below) did a litter cleanup on the access road behind V-DOT.
Among the regular trash items, they found over a hundred glass bottles,
a tire, and an old street sign.
Later on that day, Colin Nickerson, Nay Oo, Cody Murphey, and Jackson
Parker (pictured below) headed over to Briery Creek Wildlife Management
Area, just south of the H-SC campus. They cleaned up trash around the
dock, main parking lot area, and nearby walking trail.