{"id":91,"date":"2010-03-03T20:45:05","date_gmt":"2010-03-03T20:45:05","guid":{"rendered":"http:\/\/blogs.hsc.edu\/biology\/?p=91"},"modified":"2023-09-26T14:34:06","modified_gmt":"2023-09-26T14:34:06","slug":"immunology-students-use-macs-technology","status":"publish","type":"post","link":"https:\/\/blogs.hsc.edu\/biology\/2010\/03\/03\/immunology-students-use-macs-technology\/","title":{"rendered":"Immunology Students Use MACS Technology"},"content":{"rendered":"

Students currently enrolled in Biology 401 – Immunology are conducting research on the induction of dendritic cell maturation by Toll-like receptor ligands.\u00a0 A major theme throughout the Immunology course is the phenomenon of antigen-antibody interactions, including their applications to many areas of immunologic research.\u00a0 During the last two weeks of laboratory work,\u00a0Dr. Kristian Hargadon has introduced students to MACS technology.\u00a0 MACS, or magnetic activated cell sorting, is a widely used technique in many immunology research laboratories that allows one to isolate specific populations of cells from a complex mixture of cells.\u00a0 The technology relies on the use of\u00a0antibodies that recognize molecules uniquely expressed by certain cell types.\u00a0 These antibodies are coupled to magnetic beads and bind to\u00a0a single cell type in\u00a0a mixture of diverse cells.\u00a0\u00a0Following this antigen:antibody interaction, the mixture of cells is placed in a column within a\u00a0magnetic field, where only those cells bound by the magnetic antibody are retained.\u00a0 Unwanted cells pass through the column, leaving one with the desired cell type in a very pure form.\u00a0\u00a0At H-SC, Immunology students are using this technology to isolate dendritic cells from the mouse spleen.\u00a0 While this organ contains anywhere from 100-200 million cells, including B cells, T cells, macrophages, dendritic cells, etc., the students have successfully isolated 4-6 million dendritic cells per spleen in a highly pure population from this mixture.\u00a0 Upon isolating these cells, students are designing their own research projects to analyze the behavior of these splenic dendritic cells in response to exposure to Toll-like receptor ligands.\u00a0 We will keep you posted on their progress!<\/p>\n

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\"Professor<\/a><\/dt>\n
Dr.\u00a0Hargadon demonstrating MACS technology to Immunology students<\/dd>\n<\/dl>\n
\"A<\/a> <\/p>\n
<\/a>\u00a0<\/dt>\n
A MACS Cell Separator<\/dd>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Students currently enrolled in Biology 401 – Immunology are conducting research on the induction of dendritic cell maturation by Toll-like receptor ligands.\u00a0 A major theme throughout the Immunology course is the phenomenon of antigen-antibody interactions, including their applications to many … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-91","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/posts\/91","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/comments?post=91"}],"version-history":[{"count":1,"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/posts\/91\/revisions"}],"predecessor-version":[{"id":2283,"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/posts\/91\/revisions\/2283"}],"wp:attachment":[{"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/media?parent=91"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/categories?post=91"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.hsc.edu\/biology\/wp-json\/wp\/v2\/tags?post=91"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}