Dr. Kristian M. Hargadon ’01 Publishes Major Review Article on Dendritic Cells

Elliott Assistant Professor of Biology, Dr. Kristian M. Hargadon ’01, recently had a major review article published in the journal International Reviews of Immunology.  The article, entitled “Murine and Human Model Systems for the Study of Dendritic Cell Immunobiology,” highlights the tools and strategies employed by immunologists to study dendritic cells, a key regulatory cell type of the immune system that is critical for both the induction of immune activation and tolerance.  These cells play major roles in immunity to pathogens, transplant acceptance/rejection, autoimmunity, and anti-tumor immunity, and their impact on the field was the basis for the 2011 Nobel Prize in Physiology or Medicine that was awarded to three investigators who discovered and offered in-depth functional characterization of these cells.  In addition to emphasizing methodologies that have enabled experimental analyses of dendritic cells, Dr. Hargadon’s review also offers insights as to how the model systems currently in use to study these cells might be manipulated going forward to gain better a better understanding of the development and function of dendritic cells.  International Reviews of Immunology is published by Informa Healthcare and is one of the leading review journals in the field of immunology.  Dr. Hargadon’s research program focuses on the modification of dendritic cell function by tumors and how tumor-altered dendritic cells impact the quality of anti-tumor T cell responses.

H-SC biology students showcase their summer research work at C-Day

Hampden-Sydney celebrated the start of the new academic year with C-Day, a day marked by the College’s opening convocation as well as events for each of the four classes.  C-Day was also an opportunity for 10 biologists to show the results of their summer research work at a poster session held during the community picnic on campus.  Of these 10 biologists, 9 worked directly with members of the H-SC biology department on campus over the summer while the tenth, Daniel Osarfo-Akoto ’15, did his work at Harvard Medical School in conjunction with the Howard Hughes Medical Institute Exceptional Research Opportunities Program.  Many of these projects will continue in the coming academic year as students pursue these projects for academic credit and, in many cases, the production of an honors thesis.

Jay Brandt '15

Jay Brandt ’15

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Davis Carter ’15

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Kyle Deivert ’16

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Joshua Dimmick ’15

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Grayland Godfrey ’15

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Erik Kellogg ’15

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Sean Kellogg ’15

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Daniel Osarfo-Akoto ’15

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Harold Willis ’16

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Stephen Woodall ’15

 

H-SC student-generated bacteriophage sequence published on GenBank

Mycobacteriophage Cheetobro, discovered on the Hampden-Sydney campus and named by Drew Whitt ’11 and analyzed by James Hughes ’14 and Francis Polakiewicz ’14, was recently published on GenBank.  GenBank is the genomic sequence repository of the National Library of Medicine (NLM), a subsidiary of the National Institutes of Health (NIH). Cheetobro joins Arturo as the second mycobacteriophage sequence published through the work of Hampden-Sydney students in conjunction with the Howard Hughes Medical Institute (HHMI) Science Education Alliance-Phage Hunters Exploring Genomics and Evolutionary Science (SEA-PHAGES) program, a collaboration between HHMI and ~80 American colleges and universities across the country.  This fall, a new crop of students will enter the program through the Biology Department’s Molecular and Cellular Biology class and will work with bacterial hosts from genus Bacillus to further contribute to understanding virus evolution.

The link to Cheetobro may be found at: http://www.ncbi.nlm.nih.gov/nuccore/670140156

See the complete collection of mycobacteriophages isolated at Hampden-Sydney at: http://phagesdb.org/institutions/HMSD/

Electron micrograph of mycobacteriophage Cheetobro

Electron micrograph of mycobacteriophage Cheetobro

Summer explorations at Case Western Reserve University of a career in medicine

By Myshake Abdi ’16

Over the summer I was fortunate enough to spend six weeks in Cleveland, OH participating in the Summer Medical/Dental Education Program. There are multiple SMDEP sites all over the U.S. at varying dental and medical schools; the site I chose to attend was centered in the Case Western Reserve University School of Medicine.

At home at CWRU

At home at CWRU

Over the course of six weeks I had the privilege of attending multiple lectures, ranging from different groundbreaking studies in the medical field to insight on the process of applying to med school. Aside from guest lecturers we also were required to attend class. Each student was assigned a math, writing, physics, and chemistry course, and the classes we were placed in were contingent upon the classes we had already taken during the school year. In addition to class work, every student was assigned a public health group. Over the course of the program these public health groups were responsible with finding and proposing practical solutions to public health disparities, then presenting their topics in the form of a 20 to 30 page paper as well as a power point presentation.

Public Health Group

Myshake (3rd from right) with his public health group

However, the main experience that makes this program unique is the shadowing experience. Each SMDEP student, at the CWRU site, was given the ability to shadow 3-4 doctors in fields such as neurosurgery, plastic surgery, internal medicine, neurocritical care, and emergency care. This, in my opinion, was perhaps the one of the more useful experiences, in terms of understanding the day to day procedure of different doctors.

 

For more information about the program visit the page: http://smdep.org/

Or contact me at: AbdiM16@hsc.edu

Drake Bishop ’14 Received Prestigious Middleton Scholarship for Medical School

Drake Bishop, a recent graduate in H-SC’s Class of 2014, has been selected as the recipient of the 2014 Dorothy Middleton Memorial Scholarship at Eastern Virginia Medical School.  This prestigious award is given to one student from each year’s entering class of medical school and provides a full scholarship that is renewable for all 4 years of medical school.  Since its establishment in 2011, two H-SC students have received the award (Barron Frazier ’12 was the recipient in 2012).  Drake, a biology major and Summa Cum Laude graduate with Departmental Honors at H-SC, received the Samual S. Jones Phi Beta Kappa Award for Excellence in Research for his Senior Honors Project on melanoma-associated suppression of dendritic cells.  Since graduating, Drake has been a participant in the Children’s Hospital of the King’s Daughters Summer Scholar Program at Eastern Virginia Medical School, where he has conducted research in the laboratory of Dr. Amy Tang on regulation of the K-RAS signaling pathway in lung cancer.  He will begin his first year of medical school at EVMS this fall!

A summer internship in reproductive medicine

by Travis Goodloe ’16

I have a summer research internship at the Center for Reproductive Medicine here in Mobile working with Dr. George Koulianos, MD and Dr. Suzanne Degelos, Ph.D.  The Center specializes in many facets of reproductive medicine including several methods of in vitro fertilization as well as intrauterine insemination (IUI) in addition to general reproductive healthcare for couples and patients facing reproductive difficulties or infertility.  My internship involves working as a lab tech with lab  director Dr. Degelos by assisting in egg retrieval and embryo transfer procedures along with intracytoplasmic sperm injection (ICSI), which is the method used to fertilize eggs in vitro in the lab.  Along with my lab tech responsibilities, I have been given my own project involving some data analysis and bioinformatics in order to help the Center publish an abstract that they have been trying to get out for several months now.

In the IVF world, a new technology known as physiological intracytoplasmic sperm injection (PICSI) has emerged since about 2012 which utilizes a special dish coated in media containing the protein hyaluronan.  All normal morphological and functional  sperm have hyaluronan binding receptors on their heads while eggs possess hyaluronan protein on their outer surface that bind during natural fertilization.  Thus, PICSI dishes possess hyaluronan that allows for sperm binding in the dish and thus an embroylogist to select the best quality sperm for injection.  My project has included analyzing all the patient records since 2012 when PICSI was first used here at the Center and comparing pregnancy outcomes to ICSI patients from the same time period.  Many fertility clinics across the country are moving to this new method but unfortunately the sample size here at the Center as well as in other studies from around the country are not large enough to produce statistically significant results that prove PICSI is more effective and provides higher clinical pregnancy rates and more importantly successful delivery rates than standard ICSI. 

To learn more about the science behind my project, check out http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545641/

An H-SC student heart surgery internship with an H-SC alumnus

by Evan Harris ’16

I am currently participating in a six week summer internship with the East Carolina Heart Institute under the supervision of Dr. W. Randolph Chitwood ’72, the founder and director of the Institute.  Aaron Gilani ’15 will work with Dr. Chitwood in July.  In this internship, I observe and shadow Dr. Chitwood and several other doctors who work under him or alongside him.  Whether it’s through clinics, labs, or OR cases, I am directly exposed to the everyday work of thoracic surgeons, cardiovascular surgeons, and other physicians who deal with thoracic issues.  I witness open heart valve repair/replacement surgery, robot assisted minimally invasive valve repair/replacement surgery, and other thoracic surgeries.  This internship allows students to catch surgeons in action while asking questions regarding anatomy, physiology, or just the everyday struggles and rewards of being a physician.  I recommend that any student with an interest in pursuing a career in medicine should apply for this amazing opportunity.  Dr. Chitwood and his staff love to teach students, and Dr. Chitwood loves his alma mater, Hampden-Sydney College.

Alumni connections: Evan Harris '16 with Dr. W. Randolph Chitwood '72, founder and director of the East Carolina Heart Institute

Alumni connections: Evan Harris ’16 with Dr. W. Randolph Chitwood ’72, founder and director of the East Carolina Heart Institute

 

The Da Vinci surgical system used in the labs at ECU robotics lab

The Da Vinci surgical system used in the labs at ECU robotics lab

Jay Brandt ’15 Conducting Cancer Research at H-SC

Rising senior Biology major Jay Brandt has been hard at work this summer conducting cancer research in collaboration with Elliott Assistant Professor of Biology Dr. Kristian M. Hargadon ’01.  Through funding from the Virginia Foundation for Independent Colleges and the Hampden-Sydney College Honors Council, Jay has been investigating how melanoma-altered dendritic cells influence the quality of cytotoxic T cell activation.  Using dendritic cells that have been isolated from the spleens of mice, Jay has utilized an ex vivo cell culture system in which dendritic cells whose maturation and activation have been modulated by melanoma-derived factors are used to stimulate naïve CD8+ T cells.  Jay has then evaluated T cell activation using a variety of flow cytometric readouts that include CFSE-based T cell proliferation assays and intracellular cytokine staining assays.  He is also in the process of conducting ELISAs to measure T cell production of the cytotoxin granzyme B.  Collectively, these studies will provide significant insights into the induction of anti-tumor immune responses.  Jay will be attending medical school following his graduation in 2015, and he has already been accepted to Eastern Virginia Medical School through the College’s Early Assurance Program with the medical school!

Jay Brandt '15 running samples on the Biology Department's new flow cytometer!

Jay Brandt ’15 running samples on the Biology Department’s new flow cytometer!

 

H-SC biologists/football players research helmets and concussions

Harold Willis ’16

This summer I am researching head and helmet impacts that can cause concussions in American Football (Football). Through a series of experiments I will obtain data on the intensity and location of head impacts an Offensive Lineman and Defensive Lineman may suffer. Once the analysis of the data is completed, I will be able to conclude which areas of an Offensive and Defensive lineman’s brain are most susceptible to trauma. Through the data I will monitor the frequency and intensity of impact on the areas of the brain that are most susceptible and then utilize that information to focus on protection for the areas of the brain affected. To obtain this data an accelerometer is enclosed inside a ballistic gel brain model and is used to collect the data needed for my study.  This model gives me the ability to measure the intensity of the impact not only on the skull, but also on the brain. A drop test was conducted on a grass field from the average height of an offensive lineman (6’4”), and a defensive lineman (6’2”), in order to collect the intensity of impact through the accelerometers inside the brain. Once the data was collected, I placed the skull model on a variety of different helmets and ran the same experiment. This data will reveal which helmet type is best to protect the brain. From this data I will understand the susceptible areas of the brain. Later on, I hope to create a prototype for a helmet that protects Offensive and Defensive lineman from concussion brain trauma. As a college football player I am learning ways to extend my career in football and understanding how to protect football players from sport related injuries. I believe my research will impact the game of football and contribute to the protection other athletes can establish for themselves to prevent a possible concussion. I am passionate about the sport of football and envision these results helping me build a career in injury prevention. I foresee extending my focus of injury prevention to creating a helmet that protects all positions on the football field and then move to working on protection for other types of injuries that a football player or other types of athletes may sustain during their career.

 

Willis and his test subject, a skull model with ballistic gel and an accelerometer.

Willis and his test subject, a skull model with ballistic gel and an accelerometer.

Kyle Deivert ’16

This summer I have been researching head/helmet impacts in wide receivers and running backs. I have obtained data of intensity and location of head impacts for wide receivers (WRs) and running backs (RBs). After analysis of the data I have come to a conclusion of which areas of the brain are most vulnerable for each position: back, front, or side. The frequency and intensity of impacts to the specific areas of the head will determine the amount of focus on that area as far as methods of protection. After obtaining and analyzing this data I have set up the head/helmet testing. To obtain data for testing I have used accelerometers to measure intensity of impact and a synthesized ballistic gel to represent the brain in a skull model. Later in my research I hope to compare the effects of impact on different locations of an assortment of helmet brands to determine which helmets protect which areas of the brain more effectively. I have preformed dropped tests from an averaged height of 6 feet onto a grass field and collected data with the accelerometers inside the brains. I hope, at some point, to be able to move on to the design and construct new helmets for both RBs and WRs based on their more vulnerable locations of impact. As a collegiate football player I am very passionate about the sport and I am determined to achieve the best, most significant results. I hope to gather enough data to continue in the field of injury prevention and quite possible change the layout and construction of football helmets so players from youth to professional levels can play the game they love with less worry of receiving traumatic head injuries.

Deivert and Willis prepare the model for its next series of tests

Deivert and Willis prepare the model for its next series of tests

H-SC student researchers present their findings at the Howard Hughes Medical Institute

Joshua Dimmick ’15 and Grayland Godfrey ’15 represented Hampden-Sydney’s 2013-14 bacteriophage research class at the annual meeting of the Howard Hughes Medical Institute (HHMI) Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) symposium.  The SEA-PHAGES program unites ~80 institutions across the United States in the common goal of better understanding how bacteriophages have evolved over time (Learn more at http://www.hhmi.org/programs/science-education-alliance and http://www.phagesdb.org).  The symposium was held at HHMI’s state of the art Janelia Farm Research campus in Ashburn, Virginia.

Josh Dimmick, Grayland Godfrey, and H-SC project advisor Dr. Mike Wolyniak

Josh Dimmick, Grayland Godfrey, and H-SC project advisor Dr. Mike Wolyniak

Josh and Grayland presented their genomic characterization of McFly, a bacteriophage of the bacterial species Mycobacterium smegmatis originally discovered on the H-SC campus in 2012 and named by Seth Ayers ’13, a past project participant.  McFly resembles several bacteriophages isolated from across the U.S. but also has unique features such as the code for three tRNA molecules.  By studying McFly along with the phages found at other schools, undergraduates like Josh and Grayland are contributing to authentic research spearheaded by a research group at the University of Pittsburgh that regularly disseminates findings through publication in peer-reviewed scientific journals.

Presentation day!

Presentation day!

 

Talking to multiple colleagues

Talking to multiple colleagues

Josh and Grayland received an Honorable Mention award at the end of the symposium, placing their poster presentation in the top 10 of over 70 student posted presented and placing them in the same tier as students from Johns Hopkins, Brown, and Carnegie-Mellon Universities.  The SEA-PHAGES project will once again be in place at H-SC in the fall as a new group of biology students enrolled in Dr. Wolyniak’s Molecular and Cellular Biology course will attempt to isolate bacteriophage from the environment that target several species of the bacterial genus Bacillus.